It is commonly known that the properties of Gold nanoparticles depend strongly upon their size and shape. Our Gold nanoparticles are exhaustive characterized (TEM, DLS, UV-Vis, Z-potential measurements), possess high monodispersity (CV<15%) and great stability.

We offer you one of the smallest possible gold probes (0.8nm – 3nm), ideal for ultra-high-resolution EM applications. They have been extensive characterized (TEM, UV-Visible spectroscopy and DLS data), demonstrating a narrow size distribution.

TEM Microscopy

Intrinsic fluorescence

We commercialize high quality Gold nanostars, formed by multiple branches with sharp tips with highly-competitive characteristics guarantying to meet specifications.

TEM Microscopy

We offer Silver nanoparticles that can be used in applications related to their anti-microbial properties, high electrical conductivity, and optical properties. We produce the smallest PEGylated silver nanoparticles in the market

Magnetic particles on the nanoscale behave as superparamagnetic leading to particles with much higher magnet susceptibilities than in traditional paramagnets. We have developed unique magnetic nanoparticles in the market, covering them with gold, providing all the properties of the gold (inertness, protection against oxidation, easy to conjugate…) besides to magnetism.

First kit that allows ORIENTED conjugation of antibodies and his-tagged recombinant proteins on gold nanoparticles, on an easy and quick way. You will achieve better results than the ones obtained with other conjugation methods.

ORIENTED Bioconjugation of antibodies allows maximal antigen binding and favourable protein orientation meaning no loss of their biological activity. Our method also avoids nonspecific bindings increasing kit yield and sensibility in your assay.

Aplications

> Lateral flow
> Design of biosensors
> Bioconjugation
> Microscopy probe
> Immunogold

The all-in-one solution for an easy conjugation of your oligonucleotides (10 and 100 bases) to high quality nanoparticles. This kit guarantees the ORIENTED conjugation of your oligonucleotide to high quality gold nanoparticles via a thiol group chemically introduced to either its 5´- or 3´-end.

APLICATIONS

> Probes in DNA-based biosensing assays 

Easy-to-use magnetic nanoparticle conjugation kit for antibodies. The kit guarantees the ORIENTED covalent immobilization of IgG antibodies of your own choice to 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

Easy-to-use magnetic nanoparticle conjugation Kit for proteins. The kit guarantees the covalent immobilization of a protein of your own choice to carboxylic groups of 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

This kit is thought to easily modify your antibodies with oligonucleotides with length varying between 10 and 100 bases preserving their biological activity. With OligoLink kit ANTIBODY you will obtain highly-purified oligonucleotide-antibody conjugates in easy and rapid way, ready to be used in a broad range of applications such as ImmunoPCR, flow cytometry, western blotting, ELISA, etc

APLICATIONS

> Immuno-PCR
> PLA (Proximity Ligation Assay)
> PEA (Proximity Extension Assay)
> ECPA (Electrochemical Proximity Assay)

APLICATIONS

> Biosensors design
> Surfaces functionalization
> Chips biofunctionalization
> Lateral flow test
> Magnetic separation

Determination of the colloidal stability and aggregation in biological media such as saline solution, culture media, cell extracts, whole-blood or serum. 

Quantification of endotoxin units with different methods based on the use of Limulus Amebocyte Lysate -LAL. Preliminary screening, quantification, inhibition and enhancement.

Quantification of colony-forming units of aerobic bacteria, yeast and mold.

Selection of suitable cell lines according to the administration route, biodistribution and expected clearance.

Cell viability assay: MTS in metabolically active cells as indicator of proliferation. Discrimination of viable and non‐viable cells using propidium iodide and trypan blue. xCELLigence system for label-free and real-time monitoring of cell -viability.

Apoptosis: Annexin V / propidium iodide, caspase 3 and caspase 8 activation by flow cytometry Reactive Oxygen Species: determination of reactive oxygen species by flow cytometry.

Immunomodulation in PBMCs (peripheral blood mononuclear cells), macrophages and cell lines.

Phagocytosis: Functional evaluation and microscopy monitoring of nanoparticle internalization by phagocytic cells.

Inflammasome: Caspase-1 activation by flow cytometry and induction of IL-1β by enzyme immunoassay (ELISA).

Leukocyte proliferation: Clonal proliferation of PBMCs measured by flow cytometry.

Immunological response: Inflammatory cytokine profile and Th1, Th2, Th17 response of PBMCs measured by flow cytometry or Luminex multiplex technology.

Complement activation: Identification in serum of C3/C3b by western-blot. Quantification of C3b by enzyme immunoassay (ELISA).

Determination of contact properties of the nanomaterials with blood cells or plasma proteins.

Coagulation: intrinsic, extrinsic and common coagulation pathways are assessed by partial thromboplastin time (APTT) prothrombin time (PT) and Thrombin time (TT), respectively. Based on free interference Viscosity Detection System (VDS).

Haemolysis: colorimetric measurement of haemoglobin released.

Platelet activation: activated platelets detected by flow cytometry and aggregation by microscopy.

Measurement of the immune response IgG and IgM to a foreign antigen in rats treated daily with or without a sample. Histological report of organs, blood chemistry and hemogram.

Biodistribution, acute toxicity and maximum tolerated dose (OECD 423). Necropsy report of animals and collection of organs showing abnormalities.

Nanoimmunotech offers different potential formats of HEATSENS® technology for diverse customer needs.

Along these years, we have developed a bioconjugation knowhow to provide our customers the best solution for their projects, meaning that nowadays we also give you the possibility to design multifunctional structures for more accurate outcomes.

These biosensors can be applied to a broad range of sectors and applications, from the development of point-of-care devices for their use in a hospital o directly at the patient home in the clinical diagnosis field, to its use in a chicken farm for the detection of pathogenic microorganisms, or the detection of contaminants in water, and much more.

If your company needs to increase its medical device portfolio or have discover a specific biomarker that needs to be detected in very low quantities, we will be happy to introduce you our device.

For more information:

> Visit the project specific website: heatsens.com

> Contact: sales@nanoimmunotech.es

The physical and chemical properties of magnetic nanoparticles largely depend on the synthesis method and chemical structure. The 25nm magnetic nanoparticles are highly monodisperse nano-octahedrons of magnetite capped with citrate anions. These nanoparticles can be employed as platforms for many applications such as magnetic separation, hyperthermia, biosensors.

CITRATE STABILIZED:

Attachment of (bio)molecules containing thiols groups –SH.

CARBOXILATED :

Can be activated by EDC and NHS to form an intermediate that can subsequently react with primary amine groups on a specific protein or any other ligand to be conjugated.

APPLICATIONS:

> Magnetic separation
> Biosensing
> Magnetic hyperthermia
> Drug delivery